\n
\u4e0a\u6b21\u548c\u5927\u5bb6\u7b80\u5355\u7684\u8c08\u4e86\u4e0b\u4ec0\u4e48\u662f\u68c0\u9a8c\u79d1\u4ee5\u53ca\u68c0\u9a8c\u79d1\u7684\u4f5c\u7528\u3002\u4eca\u5929\u4e3b\u8981\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u68c0\u9a8c\u79d1\u5e38\u89c1\u7684\u4e00\u4e9b\u8bbe\u5907\u53ca\u4f5c\u7528\uff0c\u5e0c\u671b\u5bf9\u5927\u5bb6\u6709\u6240\u5e2e\u52a9\u3002<\/p>\n
\u4e00\uff0e\u4e34\u5e8a\u68c0\u9a8c\u5ba4<\/p>\n
1.1 \u4e09\u5206\u7c7b\u6216\u4e94\u5206\u7c7b\u5168\u81ea\u52a8\u8840\u7403\u5206\u6790\u4eea\uff1b<\/p>\n
(\u4e34\u5e8a\u610f\u4e49)\u8840\u7403\u5206\u6790\u4eea\u80fd\u591f\u5bf9\u7ea2\u7ec6\u80de\u3001\u8840\u5c0f\u677f\u3001\u767d\u7ec6\u80de\u8fdb\u884c\u51c6\u786e\u7684\u6d4b\u5b9a\uff0c\u63d0\u4f9b\u5404\u79cd\u5f02\u5e38\u62a5\u8b66\u4fe1\u606f\uff0c\u6709\u6548\u7684\u5e2e\u52a9\u533b\u751f\u7b5b\u67e5\u5f02\u5e38\u6837\u672c\uff0c\u63d0\u4f9b\u53ef\u9760\u7684\u8bca\u65ad\u4f9d\u636e<\/p>\n
1.2 \u5168\u81ea\u52a8\u5c3f\u6db2\u5206\u6790\u4eea\uff1b<\/p>\n
(\u4e34\u5e8a\u610f\u4e49)\u5c3f\u6db2\u5206\u6790\u4eea\u662f\u6d4b\u5b9a\u5c3f\u4e2d\u67d0\u4e9b\u5316\u5b66\u6210\u5206\u7684\u81ea\u52a8\u5316\u4eea\u5668\uff0c\u5b83\u662f\u533b\u5b66\u5b9e\u9a8c\u5ba4\u5c3f\u6db2\u81ea\u52a8\u5316\u68c0\u67e5\u7684\u91cd\u8981\u5de5\u5177\u3002\u5c3f\u6db2\u5206\u6790\u4eea\u5e38\u4f9d\u6d4b\u8bd5\u9879\u76ee\u5c06\u5176\u5206\u4e3a\u4e8c\u7c7b\uff1a\u2460\u4e3b\u8981\u7528\u4e8e\u521d\u8bca\u75c5\u4eba\u53ca\u5065\u5eb7\u68c0\u67e5\u4f7f\u7528\u76848-11\u9879\u7b5b\u9009\u7ec4\u5408\u5c3f\u8bd5\u5e26\u3002\u2461\u4e3b\u8981\u7528\u4e8e\u5df2\u786e\u8bca\u75be\u75c5\u7684\u7597\u6548\u89c2\u5bdf\uff0c\u5982\u80be\u75be\u60a3\u53ef\u7528PH\u3001\u86cb\u767d\u3001\u9690\u8840\uff08\u7ea2\u7ec6\u80de\uff09\u7ec4\u5408\u8bd5\u5e26\uff1b\u7cd6\u5c3f\u75c5\u7528PH\u3001\u7cd6\u3001\u916e\u4f53\u7ec4\u5408\u8bd5\u5e26\uff1b\u809d\u75c5\u60a3\u8005\u7528\u80c6\u7ea2\u7d20\u3001\u5c3f\u80c6\u539f\u7ec4\u5408\u8bd5\u5e26\u3002<\/p>\n
1.3 \u5168\u81ea\u52a8\u5c3f\u6c89\u6e23\u5206\u6790\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u91c7\u7528\u663e\u5fae\u56fe\u50cf\u5168\u81ea\u52a8\u8bc6\u522b\u6280\u672f\u5bf9\u5c3f\u6db2\u4e2d\u7684\u6709\u5f62\u6210\u4efd\u8fdb\u884c\u81ea\u52a8\u5b9a\u4f4d\u53ca\u6355\u6349\uff0c\u901a\u8fc7\u5f62\u6001\u5b66\u65b9\u6cd5\u5bf9\u5c3f\u6db2\u4e2d\u7684\u6709\u5f62\u6210\u5206\u8fdb\u884c\u81ea\u52a8\u8bc6\u522b\u548c\u5206\u7c7b\u8ba1\u6570\u7684\u4e00\u79cd\u5e38\u89c4\u68c0\u9a8c\u8bbe\u5907\u3002\u5bf9\u6ccc\u5c3f\u7cfb\u7edf\u75be\u75c5\u7684\u8bca\u65ad\u548c\u9274\u522b\u8bca\u65ad\u3001\u89c2\u5bdf\u75c5\u60c5\u3001\u5224\u65ad\u9884\u540e\u6709\u91cd\u8981\u7684\u610f\u4e49\u3002<\/p>\n
1.4 \u5168\u81ea\u52a8\u8840\u6c14\u5206\u6790\u4eea<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u662f\u4e00\u79cd\u7528\u4e8e\u8840\u6c14\u3001\u8840\u6c27\u6d4b\u8bd5\u7684\u4eea\u5668\u3002\u5229\u7528\u7535\u6781\u5728\u8f83\u77ed\u65f6\u95f4\u5185\u5bf9\u52a8\u8109\u4e2d\u7684\u9178\u78b1\u5ea6(pH)\u3001\u4e8c\u6c27\u5316\u78b3\u5206\u538b(PCO2)\u548c\u6c27\u5206\u538b(PO2)\u7b49\u76f8\u5173\u6307\u6807\u8fdb\u884c\u6d4b\u5b9a\u7684\u4eea\u5668\u3002<\/p>\n
1\u3001\u786e\u5b9a\u547c\u5438\u8870\u7aed\u7684\u7c7b\u578b\u548c\u7a0b\u5ea6<\/p>\n
2\u3001\u5224\u65ad\u9178\u78b1\u5e73\u8861\u5931\u8c03\u7c7b\u578b\u548c\u7a0b\u5ea6<\/p>\n
\u4e8c\uff0e\u751f\u5316\u5b9e\u9a8c\u5ba4<\/p>\n
2.1 \u5168\u81ea\u52a8\u751f\u5316\u5206\u6790\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u4e3b\u8981\u7528\u4e8e\u4e34\u5e8a\u9a8c\u8840\u5e38\u89c4\u3001\u5fc3\u808c\u9176\u8c31\u3001\u8840\u7cd6\u3001\u8840\u8102\u3001\u809d\u529f\u3001\u80be\u529f\u7b49\u5e38\u89c4\u751f\u5316\u6307\u6807\u7684\u68c0\u6d4b\uff0c\u5177\u6709\u91cd\u8981\u7684\u4e34\u5e8a\u8bca\u65ad\u548c\u6cbb\u7597\u610f\u4e49\u3002<\/p>\n
2.2 \u5168\u81ea\u52a8\u7535\u6cf3\u4eea<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u7528\u4e8e\u8840\u6e05\u86cb\u767d\u3001\u8102\u86cb\u767d\u3001\u8840\u7ea2\u86cb\u767d\u3001\u5c3f\u86cb\u767d\u7684\u5206\u79bb\u548c\u5206\u6790\u3002<\/p>\n
\u8fd8\u6709\u79bb\u5fc3\u673a\uff0c\u5236\u6c34\u673a\uff0c\u51b0\u7bb1\u7b49\u4e00\u4e9b\u5e38\u7528\u8bbe\u5907\u3002<\/p>\n
\u4e09\uff0e\u5fae\u751f\u7269\u5b9e\u9a8c\u5ba4<\/p>\n
3.1 \u5168\u81ea\u52a8\u8840\u57f9\u517b\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u5b83\u662f\u7528\u4e8e\u68c0\u9a8c\u8840\u6db2\u6837\u672c\u4e2d\u6709\u65e0\u7ec6\u83cc\u5b58\u5728\u7684\u4e00\u79cd\u5fae\u751f\u7269\u68c0\u6d4b\u4eea\u5668\u3002\u68c0\u6d4b\u60a3\u8005\u8840\u6db2\u4e2d\u662f\u5426\u6709\u7ec6\u83cc\u751f\u957f\u4ee5\u660e\u786e\u8bca\u65ad\u6709\u5341\u5206\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u4f7f\u4e34\u5e8a\u6709\u6548\u6cbb\u7597\u7684\u5173\u952e\u3002<\/p>\n
3.2 \u5168\u81ea\u52a8\u7ec6\u83cc\u57f9\u517b\u9274\u5b9a\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u4e0e\u7ec6\u83cc\u9274\u5b9a\u677f\u914d\u5957\u7528\u4e8e\u9274\u5b9a\u5e38\u89c1\u7684\u3001\u4ece\u4e34\u5e8a\u6807\u672c\u4e2d\u63d0\u53d6\u7684\u3001\u9769\u5170\u6c0f\u9633\u6027\u9700\u6c27\u83cc\u682a\u3001\u5948\u745f\u6c0f\u83cc\u3001\u55dc\u8840\u6746\u83cc\u3001\u80a0\/\u975e\u53d1\u9175\u83cc\u548c\u538c\u6c27\u83cc\u7b49\u83cc\u682a\u7684\u7eaf\u57f9\u517b\u7269\u3002\u5bf9\u7ec6\u83cc\u8fdb\u884c\u6b63\u786e\u7684\u5224\u5b9a\uff0c\u533a\u522b\u7ec6\u83cc\u79cd\u7c7b\uff0c\u6307\u5bfc\u4e34\u5e8a\u63d0\u524d\u6cbb\u7597\u3002<\/p>\n
3.3 \u4e8c\u6c27\u5316\u78b3\u57f9\u517b\u7bb1\uff1b<\/p>\n
(\u4e34\u5e8a\u610f\u4e49)\u5176\u901a\u8fc7\u5728\u57f9\u517b\u7bb1\u7bb1\u4f53\u5185\u6a21\u62df\u5f62\u6210\u4e00\u4e2a\u7c7b\u4f3c\u7ec6\u80de\/\u7ec4\u7ec7\u5728\u751f\u7269\u4f53\u5185\u7684\u751f\u957f\u73af\u5883\u5982\u6052\u5b9a\u7684\u9178\u78b1\u5ea6(pH\u503c\uff1a7.2-7.4)\u3001\u7a33\u5b9a\u7684\u6e29\u5ea6(37C)\u3001\u8f83\u9ad8\u7684\u76f8\u5bf9\u6e7f\u5ea6(95%)\u3001\u7a33\u5b9a\u7684CO2\u6c34\u5e73(5%)\uff0c\u6765\u5bf9\u7ec6\u80de\/\u7ec4\u7ec7\u8fdb\u884c\u4f53\u5916\u57f9\u517b\u7684\u4e00\u79cd\u88c5\u7f6e\u3002<\/p>\n
3.4\u751f\u7269\u5b89\u5168\u67dc\uff1b<\/p>\n
(\u4e34\u5e8a\u610f\u4e49)\u9632\u6b62\u5b9e\u9a8c\u64cd\u4f5c\u5904\u7406\u8fc7\u7a0b\u4e2d\u67d0\u4e9b\u542b\u6709\u5371\u9669\u6027\u6216\u672a\u77e5\u6027\u751f\u7269\u5fae\u7c92\u53d1\u751f\u6c14\u6eb6\u80f6\u6563\u9038\u7684\u7bb1\u578b\u7a7a\u6c14\u51c0\u5316\u8d1f\u538b\u5b89\u5168\u88c5\u7f6e<\/p>\n
\u56db\uff0e\u8840\u6813\u4e0e\u6b62\u8840\u5b9e\u9a8c\u5ba4<\/p>\n
4.1 \u5168\u81ea\u52a8\u8840\u51dd\u5206\u6790\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u5728\u6b62\u8840\u4e0e\u8840\u6813\u5206\u5b50\u6807\u5fd7\u7269\u7684\u68c0\u6d4b\u6307\u6807\u4e0e\u4e34\u5e8a\u5404\u79cd\u75be\u60a3\u6709\u7740\u5bc6\u5207\u8054\u7cfb\u3002\u540c\u65f6\u9700\u8981\u624b\u672f\u7684\u75c5\u4eba\u4e5f\u5fc5\u987b\u7528\u5168\u81ea\u52a8\u51dd\u8840\u5206\u6790\u4eea\u8fdb\u884c\u68c0\u6d4b\uff0c\u907f\u514d\u5728\u624b\u672f\u8fc7\u7a0b\u4e2d\u53d1\u751f\u5927\u51fa\u8840\u3002<\/p>\n
4.2 \u5168\u81ea\u52a8\u8840\u6d41\u53d8\u5206\u6790\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u901a\u8fc7\u68c0\u6d4b\u4eba\u4f53\u8840\u6db2\u7c98\u5ea6\u6765\u8fdb\u884c\u75be\u75c5\u65e9\u671f\u8bca\u65ad\u7684\u4e13\u7528\u68c0\u6d4b\u4eea\u5668\uff0c\u68c0\u6d4b\u4eea\u7684\u5404\u9879\u68c0\u6d4b\u6307\u6807\uff0c\u5bf9\u9ad8\u8840\u538b\u3001\u52a8\u8109\u786c\u5316\uff0c\u51a0\u5fc3\u75c5\u3001\u5fc3\u808c\u6897\u585e\uff0c\u80ba\u5fc3\u75c5\u3001\u8111\u6813\u585e\u3001\u8111\u6897\u585e\u7b49\u5fc3\u8111\u8840\u7ba1\u75c5\u3001\u5916\u5468\u8840\u7ba1\u75c5\u3001\u7cd6\u5c3f\u75c5\u3001\u80bf\u7624\u3001\u795e\u7ecf\u7cbe\u795e\u75c5\u7b49\u75c5\u56e0\uff0c\u8bca\u65ad\uff0c\u9884\u9632\uff0c\u6cbb\u7597\uff0c\u76d1\u6d4b\u90fd\u6709\u91cd\u8981\u7684\u4e34\u5e8a\u610f\u4e49\u3002<\/p>\n
\u7535\u89e3\u8d28\u5206\u6790\u4eea\uff1b<\/p>\n
4.3 \u5168\u81ea\u52a8\u8840\u6c89\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u7528\u4e8e\u5bf9\u8840\u6db2\u7ea2\u7ec6\u80de\u6c89\u964d\u7387\u7684\u6d4b\u5b9a<\/p>\n
\u4e94\uff0e\u514d\u75ab\u5b9e\u9a8c\u5ba4<\/p>\n
5.1 \u5168\u81ea\u52a8\u9176\u6807\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u4e0e\u9002\u914d\u8bd5\u5242\u914d\u5408\u7528\u4e8e\u751f\u7269\u6837\u672c\u4e2d\u5f85\u6d4b\u7269\u7684\u5b9a\u6027\u548c\u5b9a\u91cf\u5206\u6790\u3002<\/p>\n
5.2 \u6d17\u677f\u673a\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u4e00\u822c\u548c\u9176\u6807\u4eea\u914d\u5957\u4f7f\u7528\u3002\u4e3b\u8981\u7528\u4e8e\u6e05\u6d17\u9176\u6807\u677f\u68c0\u6d4b\u540e\u7684\u4e00\u4e9b\u6b8b\u7559\u7269\u8d28\uff0c\u4ece\u800c\u964d\u4f4e\u540e\u7eed\u68c0\u6d4b\u8fc7\u7a0b\u4e2d\u56e0\u6b8b\u7559\u7269\u5bfc\u81f4\u7684\u8bef\u5dee<\/p>\n
5.3 \u5168\u81ea\u52a8\u514d\u75ab\u5316\u5b66\u53d1\u5149\u5206\u6790\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u514d\u75ab\u9879\u76ee\u68c0\u6d4b\uff0c\u5e94\u7528\u4e8e\u7532\u72b6\u817a,\u6fc0\u7d20,\u5fc3\u808c\u635f\u4f24\u548c\u80bf\u7624\u6807\u5fd7\u3001\u4f20\u67d3\u75c5\u3001\u8d2b\u8840\u3001\u9aa8\u4ee3\u8c22\u7b49\u5e7f\u6cdb\u7684\u6d4b\u5b9a\u9879\u76ee\u3002<\/p>\n
\u516d\uff0e\u827e\u6ecb\u75c5\u521d\u7b5b\u5b9e\u9a8c\u5ba4<\/p>\n
6.1 \u8367\u5149\u5b9a\u91cfPCR\u68c0\u6d4b\u4eea\uff1b<\/p>\n
\uff08\u4e34\u5e8a\u610f\u4e49\uff09\u662f\u6307\u901a\u8fc7\u8367\u5149\u67d3\u6599\u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02\u6027\u63a2\u9488\uff0c\u5bf9PCR\u4ea7\u7269\u8fdb\u884c\u6807\u8bb0\u8ddf\u8e2a\uff0c\u5b9e\u65f6\u76d1\u6d4b\u53cd\u5e94\u8fc7\u7a0b\u7684\u4eea\u5668\u3002\u5176\u4f5c\u7528\uff1a\u5bf9\u81f4\u75c5\u5fae\u751f\u7269\u8fdb\u884c\u5b9a\u6027\u548c\u5b9a\u91cf\u7684\u68c0\u6d4b\uff0c\u5bf9\u81f4\u75c5\u5fae\u751f\u7269\u8fdb\u884c\u5206\u578b,\u7a81\u53d8\u682a\u5206\u6790\uff0c\u5bf9\u67d3\u8272\u4f53\u7a81\u53d8\u5bfc\u81f4\u7684\u9057\u4f20\u75c5\u7684\u68c0\u6d4b\uff0c\u764c\u57fa\u56e0\u7684\u68c0\u6d4b\uff0c\u5f25\u8865\u514d\u75ab\u68c0\u6d4b\u7684\u7f3a\u9677\uff0c\u6307\u5bfc\u7528\u836f\u8fc7\u7a0b\u53ca\u5242\u91cf,\u5e76\u5bf9\u6cbb\u7597\u8fc7\u7a0b\u8fdb\u884c\u7597\u6548\u76d1\u6d4b\u7b49\u3002<\/p>\n
\u5176\u4e2d\u8fd8\u6709\u8d85\u51c0\u5de5\u4f5c\u53f0\uff0c\u751f\u7269\u5b89\u5168\u67dc\u7b49\u5c31\u4e0d\u4e00\u4e00\u4ecb\u7ecd\u4e86\uff1b<\/p>\n
\u4e03\uff0e\u8840\u6db2\u7ec6\u80de\u5b66\u5b9e\u9a8c\u5ba4<\/p>\n
\u9ad8\u500d\u53cc\u76ee\u663e\u5fae\u955c\uff08\u5e38\u89c4\u5ba4\u3001\u7ec6\u83cc\u5ba4\u3001\u9aa8\u9ad3\u3001\u75c5\u7406\uff09\uff0c\u8d85\u9ad8\u500d\u663e\u5fae\u8bca\u65ad\u4eea<\/p>\n
\u516b\uff0e\u5176\u4ed6<\/p>\n
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